A-Z of Quantitative PCR (IUL Biotechnology, No. 5)

A-Z of Quantitative PCR (IUL Biotechnology, No. 5) by Bustin, Stephen A.. hardcover edition. ISBN: 9780963681782.

Bustin (School of Medicine and Dentistry, University of London, UK) and contributors explain features of quantitative PCR (qPCR) and provide alternative viewpoints and methods in this hands-on manual for novice and expert researchers who want to learn how to set up and carry out qPCR assays from scratch. Early chapters give insight on factors that influence a PCR experiment and principles underlying real-time quantification. The core of the book details the steps involved in conducting a qPCR experiment, from sample collection to interpretation of results. Final chapters provide a protocol for mRNA quantification and guidelines for standardization of qPCR assays. Annotation ©2004 Book News, Inc., Portland, OR (booknews.com)