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Live Cell Imaging: A Laboratory Manual

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Recent advances in imaging technology reveal in real time and great detail critical changes in living cells and organisms. This manual is a compendium of emerging techniques organized into two parts: specific methods such as fluorescent labeling and delivery and detection of labeled molecules in cells; and experimental approaches ranging from the detection of single molecules to the study of dynamic processes in organelles organs and whole animals. Although presented primarily as a laboratory manual the book includes introductory and background material and could be used as a textbook in advanced courses. It also includes a DVD containing movies of living cells in action created by investigators using the imaging techniques discussed in the book.